EXPAND HIFI PLUS PCR SYS. 500 U (C15-1302-834)

General description

The Expand High Fidelity^PLUS PCR System is a blend of Taq DNA polymerase and a thermostable proofreading protein that lacks polymerase activity.
The Expand High Fidelity^PLUS PCR System is designed to amplify fragments up to 5 kb from all types of DNA with high yield and fidelity. It amplifies DNA with sixfold greater fidelity than Taq DNA polymerase alone.
Expand High Fidelity^PLUS PCR System also incorporates dUTP. Cross-contamination can therefore be prevented by a pretreatment of dUTP containing DNA with heat-labile Uracil-DNA Glycosylase before amplification. It is suited for the simultaneous amplification of a large number of targets.

Application

The Expand^™ High Fidelity^PLUS PCR System designed for the robust amplification of fragments up to 5 kb from all types of DNA with outstanding yield, specificity, sensitivity, and accuracy. This kit is the product of choice for the development of a new amplification assay or the optimization of an existing assay.
Use Expand High FidelityPLUS PCR System for:

• PCR
• RT-PCR
• Primer extension
• Carryover prevention

Features and Benefits

• Safeguard against cross-contamination.

This is the choice for large numbers of amplifications.

• Obtain sixfold higher fidelity and higher yields,

compared to Taq DNA Polymerase.

• Efficiently label DNA.

Use this kit to label DNA with radioactive or nonradioactive nucleotides.

Packaging

1 kit containing 4 components

Quality

Each lot is PCR function-tested using human genomic DNA and EPO primers specific for a 1.8kb PCR product, and tPA primers specific for a 4.8kb PCR product.

Unit Definition

Volume Activity: 5 U/μL

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

Transition-metal-catalyzed cross-coupling is an efficient tool for constructing C-C, C-N and C-O bonds in organic synthesis. Intensive interest has been focused on the development of the most effective ligands that improve catalyst performance. Ligands play a key role in stabilizing and activating the central metal atom and fine-tuning the selectivity of transformation. Phosphines have proven to be the most significant class of ligands for cross couplings, such as Negishi, Suzuki, Stille, Heck, Sonogashira, and Buchwald-Hartwig cross-coupling.

Expand is a trademark of Roche

usage: sufficient for ≤. 2,500 . reactions (03300234001), sufficient for 2500 . reactions, sufficient for ≤. 500 . reactions (03300226001), sufficient for 500 . reactions. Quality Level: 100. feature: High Fidelity PCR, dNTPs included: no, hotstart: no. packaging: pkg of 2,500 . U (03300234001 [10x250 U]), pkg of 500 . U (03300226001 [2x250 U]). manufacturer/tradename: Roche. parameter: 72 °C optimum reaction temp.. technique(s): PCR: suitable. input: purified DNA. storage temp.: −. 20°C. Hazard Statements: H412. Precautionary Statements: P273 - P501. Hazard Classifications: Aquatic Chronic 3. Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 2. Flash Point(F): does not flash. Flash Point(C): does not flash.