FastLyse pDNA Miniprep Kit, 50 Columns

FastLyse Miniprep Kit incorporates one-step lysis technology with a uniquely formulated buffer to lyse the bacterial culture and convenient spin column technology to purify up to 25µg of pDNA in just 10 minutes. Each column contains a unique silica membrane that binds up to 50µg of plasmid DNA. This technology does away with cumbersome pellet formation and resuspension steps, phenol extraction and ethanol precipitation. FastLyse Miniprep Kits yield ultrapure pDNA with A260/A280 ratio of 1.8 - 2.0 and is suitable for downstream applications like library construction, restriction digestion, cloning, ligation, bacterial transformation, PCR, Automated sequencing, Probe Generation and Microinjection. Features Fastest pDNA isolation; in 10 minutes Works with very low culture volumes (600μl) No pellet formation and resuspension steps Very high binding capacity of up to 50μg Very high yields with low elution volumes Very high within lot and inter lot consistency Applications Plasmid Screening Restriction Digestion Cloning Ligation Bacterial Transformation PCR Automated Sequencing