Fibronectin from Bovine Plasma, 5mg

Fibronectin are adhesive glycoproteins and are found both as cell surface proteins and in plasma. They are large glycoproteins found in cells and extracellular fluids. They are involved in a wide range of biological activities including cell adhesion to extracellular matrices, blood coagulation and wound healing. In tissue culture, fibronectins promote cell adhesion and spreading on synthetic surfaces. Certain cell types show morphological changes relating to loss of fibronectin from the cell surface or depletion of fibronectin from the culture medium. Fibronectins are of two, closely related, major types: cellular fibronectin, otherwise known as Large External Transformation Sensitive (LETS) protein and plasma fibronectin, also called Cold-insoluble Globulin (CLg). Fibronectin has been shown to stimulate the proliferation of rat osteoblasts in vitro; to induce odontoblast polarization and to regulate the differentiation of human dental pulp cells into odontoblasts. It has wide applications in cellular research into proliferation, differentiation, development and morphology. Precoating of glass, plastic and micro-carrier beads aids cell attachment, particularly those cell which are deficient in cell surface fibronectin. It has been reported that proteolytic digests of fibronectin are mitogenic for CH23 fibroblasts. Domains of fibronectin bind with collagen, fibrin, heparin, and specific cell membrane receptors. The most notable domain, Arg-Gly-Asp can be recognized by integrins and mediates cell adhesion. Fibronectin is also widely involved in interactions such as the attachment and migration of many cell types, cytoskeletal assembly, tyrosine phosphorylation, and metastasis. Cell culture substratum at 1-5 μg/cm 2 or 0.5-50 μg/mL.