FIREFLY LUCIFERASE, 100 ASSAYS

Lumino™ Firefly Luciferase Glow Assay is designed for high throughput screening or quantification of luciferase expression in mammalian cells which is mainly performed by batch processing of 96- and 384- well plates. Lumino™ Firefly Luciferase Glow Assay is homogenous, high-sensitive, glow type (long-lived) bioluminescent assay with signal half-life of more than 3 hrs. A long signal half-life (>3hrs) of the luminescent assay is necessary for batch-processing (HTS) systems, where a large number of plates can be stacked after assay and before measurement. Features: High sensitivity: High sensitive detection of firefly luciferase activity. Suitable for HTS assays: Designed for batch-processing of mammalian cells. Single reagent is added for cell lysis and luminescent assay which makes HTS assays faster to perform. Stable signal: Glow type bioluminescent assay with signal half-life of greater than 3 hrs. Excellent linearity: Linear over high-log order (5 or more) of luciferase concentration. The linear range is also dependent of the sensitivity of the luminometer. Assay reagents compatible with other firefly luciferases. Application(s): Rapid and high sensitive detection or quantification of firefly luciferase reporter enzyme activity. Suitable forhigh throughput screening or quantification of luciferase expression in mammalian cells as its glow typebilumnescent assay with signal half life of more than 3 hours.