Solution I - Alcoholic Hematoxylin
Solution II - Aqueous Ferric Chloride (10%)
Solution III - Iodine
Solution IV - Aqueous Ferric Chloride (2%)
Solution V - Aqueous Phloxine (0.5%)

Used to stain elastic fibers, nuclei and collagen.

CONTENTS: Hematoxylin, Ethanol, Ferric Chloride, Iodine, Potassium Iodide, Phloxine

These solutions are made from certified dyes (when applicable).

FOR IN VITRO DIAGNOSTIC USE ONLY.

RECOMMENDED PROCEDURE: Deparaffinize through (1 part peanut oil - 2 parts xylene) 2 changes, 12 minutes each. Drain, wipe off excess oil and blot to opacity. The residual oil helps prevent shrinkage and injury of section. Sol. I - 30 minutes at room temperature. Wash in tap water - 3 minutes. Differentiate slides individually with Sol. II until sections are faint pink. Wash in tap water - 3 minutes. Sol. III - 30 seconds. Rinse in water, blot and ALLOW TO AIR DRY THOROUGHLY. Before mounting, dip slide in xylene.

RESULTS: Acid-fast bacilli - red; Lepra bacilli - red; Norcardia filaments - red; background - pale blue.

Safety Data Sheet - Fite's Method Stain Kit for Acid Fast Bacteria in Tissue (Solution I)

Safety Data Sheet - Fite's Method Stain Kit for Acid Fast Bacteria in Tissue (Solution II)

Safety Data Sheet - Fite's Method Stain Kit for Acid Fast Bacteria in Tissue (Solution III)