FKBP-8 Antibody (C15-1317-951)

General description

FKBP-8, also known as Peptidyl-prolyl cis-trans isomerase PPIase FKBP8, FKBP8, 38 kDa FK506-binding protein, 38 kDa FKBP, FKBP-38, hFKBP38, FK506-binding protein 8, FKBP-8, FKBPR38, Rotamase, is encoded by the gene name FKBP8 and FKBP38. FKBP-8 is in constitutively inactive PPiase, which becomes active when bound to calmodulin and calcium. Recent studies have shown that FKBP-8 may act as a chaperone for BCL2, by targeting BCL2 to the mitochondria and modulating its phosphorylation state. Furthermore, the BCL2/FKBP8/calmodulin/calcium complex may play a role in the binding of BCL2 to its targets. The active form of FKBP8 may therefore play a role in the regulation of apoptosis. FKBP-8 is expressed widely, with highest levels detected in brain. FKBP-8 is also highly abundant in the retina.

Immunogen

Epitope: N-terminus

Recombinant protein corresponding to the N-terminus of human FKBP-8.

Application

Research Category
Signaling

Research Sub Category
Signaling Neuroscience

This FKBP-8 antibody is validated for use in WB, IH for the detection of FKBP-8.

Western Blotting Analysis: A representative lot detected FKBP-8 in HeLa transfected with myc-FKBP38 and HEK293 cell lysate (Zou, H., et al. (2013). JBC. 1-9).
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected FKBP-8 in human cerebral cortex and human thalamus tissue.

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected FKBP-8 in 10 µg of HeLa cell lysate.

Target description

~60 kDa observed. The calculated molecular weight is 44 kDa, however FKBP-8 has been shown at ~53-62 kDa most likely due to post-translational modification.

Physical form

Rabbit polyclonal serum with 0.05% sodium azide.

Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: serum. clone: polyclonal. species reactivity: human. technique(s): immunohistochemistry: suitable, western blot: suitable. NCBI accession no.: NP_036313. UniProt accession no.: Q14318. shipped in: dry ice. target post-translational modification: unmodified. Storage Class Code: 10 - Combustible liquids. WGK: WGK 1.