Application
The FLAG Purification Kit utilizes CelLytic™ Reagents for rapid and efficient cell lysis and protein extraction and ANTI-FLAG® M2 affinity gel for affinity purification of active FLAG fusion proteins.
Browse additional application references in our FLAG® Literature portal.
Features and Benefits
- Includes ready to use reagents, columns, and a detailed protocol for affinity purification of FLAG fusion proteins.
- ANTI-FLAG M2 Affinity Gel allows efficient binding of FLAG fusion proteins without the need for preliminary steps and calibrations.
- Two alternatives for efficient elution conditions (by acidic conditions or by competition with FLAG peptide).
Packaging
Sufficient for 3-5 uses of 1-ml affinity purification column.
Other Notes
Kit contains CelLyticâ„¢ M for cell lysis and protein extraction from Mammalian cells.
Legal Information
ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC
CelLytic is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Sigma-Aldrich Co. LLC
- UPC:
- 41106510
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- CELLMM2-1KT