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fluorescein-12-dutp

FLUORESCEIN-12-DUTP

Catalog No.
C15-1297-001
Manufacturer No.
11373242910
Manufacturer Name
Sigma-Aldrich
Quantity
25
Unit of Measure
UL
Price: $517.92
List Price: $575.47

Application Fluorescein-12-dUTP is used as a substrate for: terminal transferase DNA polymerase I (holoenzyme and Klenow fragment), Taq DNA polymerase reverse transcriptase ( e.g.

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Application

Fluorescein-12-dUTP is used as a substrate for:

  • terminal transferase
  • DNA polymerase I (holoenzyme and Klenow fragment),
  • Taq DNA polymerase
  • reverse transcriptase (e.g., from AMV and M-MuLV).
Fluorescein-12-dUTP has been used to label nuclear extracts during PCR. It has also been used to replace dTTP in the random-primed DNA labeling reaction or in nick translation reactions, as well as in PCR. The nucleotide also serves as a substrate for terminal transferase in 3′-end labeling and TUNEL. Fluorescein-labeled probes can be used for in in situ hybridization with direct fluorescence detection or detection by enzyme linked immunoimmunsorbent assay (ELISA) using Anti-Fluorescein-AP, Fab fragments. Repeated fluorescence labeling using Tetramethyl-Rhodamine-6-dUTP (red) and AMCA-6-dUTP (bright blue) is possible.

Quality

Typical analysis: At least 85% Fluorescein-12-dUTP (HPLC, area%).

Physical form

Fluorescein-12-dUTP, tetralithium salt.

Storage and Stability

The unopened reagent is stable at 15 to 25°C until the expiration date printed on the label. Decomposition of approx. 5% may occur within 6 months.

Other Notes

For life science research only. Not for use in diagnostic procedures.

UPC:
51473105
Condition:
New
Weight:
1.00 Ounces
HazmatClass:
No
WeightUOM:
LB
MPN:
11373242910


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