Fluorescein-Conjugated Goat Affinity Purified F(ab')2 Fragme (C08-0505-524)

Description: Analysis Note The total protein is measured by absorbance (A)280 nm); F/P ratio is calculated using absorbance at 491.5nm. Antibody titer of the affinity purified F(ab')2 fragments is standardized with an in-house control by immunoelectrophoresis. The affinity purified F(ab')2 fragment is tested for purity at 10 mg/mL using immunoelectrophoresis. The product is goat F(ab')2; no trace of albumin, whole molecule IgG or Fc is detected. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strongly acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. Preparation Method: Antibody and FITC are conjugated under defined conditions to obtain the specified molar ratio (3-6 mol/mol). Conjugated protein is purified by gel filtration. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide and 10% glycerol (v/v), adjusted to standard titer, filtered through a 0.22 µm filter, and vialed. Applications: This product is suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. Use of affinity purified antibodies is recommended to avoid non-specific binding from the inherent antibodies of host animals; these F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. Product Description: Product is fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated goat affinity purified F(ab')2 fragments to mouse immunoglobulins (IgG,IgA,IgM) and buffer salts.