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Fluorescein-Conjugated Goat F(ab')2 Fragment to Human IgA (a

Catalog No.
C08-0505-550
Manufacturer No.
855178
Manufacturer Name
MP BIOMEDICALS
Quantity
1
Unit of Measure
EA
Price: $603.06
List Price: $670.07

The total protein is measured using the Biuret procedure with bovine albumin as the standard F/P ratio is calculated using absorbance at 491.5nm.

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The total protein is measured using the Biuret procedure with bovine albumin as the standard; F/P ratio is calculated using absorbance at 491.5nm. Antibody titer of the IgG fraction is standardized by equivalence-point precipitation. The F(ab')2 fragments are tested for purity and specificity at a minimum of 20 mg/mL using immunoelectrophoresis. The product is goat F(ab')2; no trace of albumin, whole molecule IgG or Fc is detected. Antibody and FITC are conjugated under defined conditions to obtain the specified molar ratio (3-6 mol/mol). Conjugated protein is purified by gel filtration. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 μm filter, vialed, and lyophilized. IgA antibody is a secretory antibody and is present abundantly in mucous linings of gastrointestinal, respiratory and genitourinary tracts, tears and saliva. Thus, secretory IgA contributes to the humoral defense mechanism against the pathogens on mucosal surfaces. Anti-Human IgA (α-chain specific)-FITC antibody is specific for α chain of human IgA. Goat Anti-Human IgA is conjugated to Fluorescein Isothiocyanate (FITC), Isomer I. Fluorescein-conjugated goat F(ab')2 fragment to human IgA (α chain) is used as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. Note: Affinity purified antibodies and their F(ab')2 fragments are recommended to avoid non-specific binding from the inherent antibodies of host animals. A dilution of 1:100 was used for immunohistochemistry of cryosections of human sural nerves. Fluorescein-conjugated Goat F(ab')2 fragment to Human IgA is the lyophilized powder of fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated goat F(ab')2 fragment to human IgA (α chain) and buffer salts.
UPC:
41116127
Condition:
New
Availability:
3 Days
Weight:
1.00 Ounces
HazmatClass:
No
WeightUOM:
LB
MPN:
855178


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