Fluorescein (FITC) AffiniPure F(ab')2 Fragment Rabbit Anti-Human IgG, Fcy fragment specific, 0.5 mg

F(ab')2 fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region. F(ab')2 fragments have two antigen-binding Fab portions linked together by disulfide bonds and therefore they are divalent. The average molecular weight is about 110 kDa. They are used for specific applications, such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G. Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the Fc portion of human IgG heavy chain but not with the Fab portion of human IgG. No antibody was detected against human IgM or IgA, or against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species. Target: Human Host: Rabbit Antibody Format: F(ab')₂ Fragment Specificity: IgG, Fcγ fragment specific Conjugate: Fluorescein (FITC) Clonality: Polyclonal This product is for IN VITRO RESEARCH USE ONLY. It is not a medical device and it is not intended for diagnostic or therapeutic purposes.