Cell Line Origin
Human fibrosarcoma (retroviral packaging cells)
Cell Line Description
FLYRD18 has been developed together with FLYA13 (Sigma catalog number 95091901) for packaging of retroviruses, it is especially useful for in vivo gene delivery. Both cell lines have been derived from HT1080 (Sigma catalog number 85111505). FLYRD18 has been transfected with Moloney murine leukaemia virus cores with envelope glycoproteins of cat endogenous virus RD114. This cell line is able to produce human complement-resistant retroviral vectors at titres consistently higher than 1x10,000,000i.u./ml. Addition of 4μg/ml blasticidin S and 10μg/ml phleomycin into the media may enhance the packaging function. No replication competent virus has been detected in this cell line.
DNA Profile
Not specified
Culture Medium
DMEM + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:20, i.e., seeding at 1x10,000 cells/cm2 using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37 °C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Disclaimer
This cell line has special release conditions: All customers are required to complete a Cell line Release Authorisation form.
biological source: human fibrosarcoma. growth mode: Adherent. karyotype: Not specified. morphology: Epithelial-like. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. relevant disease(s): cancer. shipped in: dry ice.- UPC:
- 42192206
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 95091902-1VL
