FMOC-ARG(PBF)-OH 25 G

General description

High purity Fmoc-protected amino acid for research and process production of peptides, with very low levels of dipeptide, free-amino acids and acetic acid impurities.
The standard derivative for the introduction of Arg in Fmoc SPPS ^[1,2]. The Pbf side-chain protecting group is removed with TFA approximately 1-2 times faster than Pmc.In the preparation of peptides containing both Arg and Trp, it is recommended that this derivative is used in conjunction with Fmoc-Trp(Boc)-OH (852050).

Associated Protocols and Technical Articles
Fmoc-amino acids for Peptide Production
Cleavage and Deprotection Protocols for Fmoc SPPS


Literature references

^[1] L. A. Carpino, et al. (1993) Tetrahedron Lett., 34, 7829.
^[2] C. G. Fields, et al. (1993) Tetrahedron Lett., 34, 6661.

Linkage

Replaces: 04-12-1145

Analysis Note

Colour (visual): white to off white
Appearance of substance (visual): powder
Colour index (0,5 M in DMF): ≤ 150 Hazen
Identity (IR): passes test
Enantiomeric purity: ≥ 99.8 % (a/a)
Purity (HPLC): ≥ 99.0 % (a/a)
Fmoc-ß-Ala-OH (HPLC): ≤ 0.1 % (a/a)
Fmoc-ß-Ala-Arg(Pbf)-OH (HPLC): ≤ 0.1 % (a/a)
Fmoc-Arg(Pbf)-Arg(Pbf)-OH (HPLC): ≤ 0.1 % (a/a)
Fmoc-Arg-OH (HPLC): ≤ 0.1 % (a/a)
Assay free amino acid (HPLC): ≤ 0.2 %
Purity (TLC(011C)): ≥ 98 %
Purity (TLC(157B)): ≥ 98 %
Solubility (12,5 mmol in 25 ml DMF): clearly soluble
Assay (acidimetric): ≥ 90.0 %
Water (K. F.): ≤ 2.0 %
Ethyl acetate (HS-GC): ≤ 1.0 %
Acetate (IC): ≤ 0.02 %

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Legal Information

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