General description
Fmoc-TOAC-OH is a useful tool for the incorporation of the ESR spin-label TOAC into peptide sequences [1] by Fmoc SPPS. Incorporation of this derivative and the following residue is best achieved using HATU activation. TFA/water/TIS should be used for cleavage of TOAC-containing peptides. The use of EDT should be avoided as it can cause permanent reduction of the nitroxide radical [2]. Following cleavage, the TOAC peptide should be treated with aqueous ammonia in air to regenerate the nitroxide from the hydroxylamine that is generated by the TFA treatment.
Associated Protocols and Technical Articles
Cleavage and Deprotection Protocols for Fmoc SPPS
Chromogenic Labeling of Synthetic Peptides
Literature references
[1] R. Marchetto, et at. (1993) J. Am. Chem. Soc., 115, 11042.
[2] L. Martin, et al. (2001) J. Pept. Res., 58, 424.
Analysis Note
Color (visual): white to yellow to beige
Appearance of substance (visual): powder
Identity (IR): passes test
Assay (HPLC, area%): ≥ 98.0 % (a/a)
Legal Information
Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany
- UPC:
- 51442007
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 8523428500
