FocusT DTT, 2M, 5Vial

FOCUS™ Cytoplasmic/Nuclear Protein Extraction (Thomas No. C994C79) Fractionation method is based on gentle lysis of the sample followed by centrifugation to collect and separate nuclei from the rest of the cytoplasmic proteins. Kit contains reagents necessary for fractionation and solubilization buffer suitable for IEF/2D gels for better spot resolution. Applications: Fractionation of cytoplasmic and nuclear proteins. Supplied with a strong chaotropic extraction buffer to solubilize both cytoplasmic as well as nuclear proteins for 2-D gel analysis. 50 preps FOCUS™ Cytoplasmic/Nuclear Protein Extraction (Thomas No. C994C80) Fractionation method is based on gentle lysis of the sample followed by centrifugation to collect and separate nuclei from the rest of the cytoplasmic proteins. Kit contains reagents necessary for fractionation and solubilization buffer suitable for IEF/2D gels for better spot resolution. Applications: Fractionation of cytoplasmic and nuclear proteins. Supplied with a strong chaotropic extraction buffer to solubilize both cytoplasmic as well as nuclear proteins for 2-D gel analysis. 50 preps Ultra-pure proteomic grade Iodacetamide for protein alkylation (Thomas No. C994C81) The kit is supplied with a proprietary buffer necessary for efficient alkylation of thiols, while minimizing reoxidation of the competing thiol pairs in protein samples. Simply add an appropriate amount to reagent solutions for alkylation of protein thiol groups. Applications: Preparation of protein samples for 2D gel electrophoresis Blocking of thiols for preventing re-oxidation of proteins FOCUS™ Protein Reductant Kit (Thomas No. C994C82) A water soluble, odorless, non-toxic, and stable TCEP (Tri-(2-carboxyethyl) phosphine) reductant for protein reduction. As compared to DTT, the TCEP reductant is more stable, more effective, and able to work over a wide pH range, including lower acidic pHs. Reduces completely even the most stable disulfide bonds in less than 5 minutes at room temperature. Use of the TCEP reductant is compatible with the alkylation reaction of the SH-groups for 2D gel analysis. 100 preps FOCUS™ Protein Reduction-Alkylation Kit (Thomas No. C994C83) Improves the resolution and prevents streaking of protein spots on 2-D gels. Reduction-Alkylation kit offers a simple two step method for reduction and alkylation of protein samples for 2D gel analysis. Disulfide bonds are reduced with a highly reactive and stable TCEP followed by blocking of the thiols by alkylation and preventing re-oxidation of proteins 100 preps FOCUS™ Signal Protein Protein Extraction Kit (Thomas No. C994C84) FOCUS™ Signal Proteins kit fractionates lipid raft localized proteins from other cellular proteins by employing non ionic detergents. Signal Protein Extraction Buffer is a proprietary formulation of non-ionic detergents designed to efficiently extract and remove soluble proteins, leaving lipid rafts containing signal proteins as a detergent insoluble fraction. The resulting rafts are then solubilized in FOCUS™ Protein Solubilization Buffer, a supplied 2D electrophoresis compatible buffer or a different buffer of choice. Applications: For fractionation of caveolin-enriched membrane proteins, proteins rich in cholesterol, glycolipids, GTP-binding, and GPI linked proteins and protein molecules involved in directing intracellular membrane traffic Suitable for 2D gel electrophoresis Suitable for proteomics and cell signalling studies 50 preps.