Folic acid assay medium

Application

Recommended for the microbiological assay of folic acid using Streptococcus faecium as the test organism.

Components

Components (g/L)
Casein Acid Hydrolysate, Vitamin Free 12.00
Dextrose 40.00
Sodium Citrate 20.00
L-Cystine 0.20
DL-Tryptophan 0.20
Adenine Sulfate 0.02
Guanine Hydrochloride 0.02
Uracil 0.02
Thiamine Hydrochloride 0.002
Pyridoxine Hydrochloride 0.004
Riboflavin (Vitamin B₂) 0.002
Nacin 0.002
p-Aminobenzoic Acid (PABA) 0.0002
Biotin 0.0000008
Calcium Pantothenate 0.0004
Dipotassium Phosphate 1.00
Monopotassium Phosphate 1.00
Magnesium Sulfate 0.40
Sodium Chloride 0.02
Ferrous Sulphate 0.02
Manganese Sulphate 0.02

Preparation Note

Suspend 7.5 g of Folic Acid Assay Medium in 100 ml of distilled water. Boil to dissolve the medium completely. Mix well to distribute the slight precipitate evenly. For assay, dispense 5 ml of medium per assay tube, and add 5 ml of distilled water to each tube. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 10 minutes. Cool immediately.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany