General description
Formamide, a naturally occurring amide, contains carbon, hydrogen, oxygen, and nitrogen in its structure.
Application
Formamide has been used:
- as a component in the hybridization buffer for in situ hybridization as a component of solution X to wash the slides to remove the coverslips
- in the preparation of denaturation solution for spectral karyotyping (SKY) analysis
- in the preparation of 1% formaldehyde solution for the pretreatment of slides for comparative genomic hybridization (CGH)
- to prepare pre-hybridization buffer I, II, III, IV, and hybridization buffer I, II, and III for RNA fluorescence in situ hybridization (RNA-FISH)
- as an ionizing solvent in the production of formic esters and vitamin, a softener for paper, in animal glues, and water-soluble gums, in molecular biology as a denaturing agent
Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.
Biochem/physiol Actions
Formamide serves as a good solvent for polar organic and inorganic compounds with potential catalytic activity in prebiotic processes due to its high dielectric constant.
Other Notes
For the preparation of formamide denaturing polyacrylamide gels; In situ hybridization on human chromosomes
- UPC:
- 41105326
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- 47671-1L-F
- CAS:
- 75-12-7
