General description
Consanguineous: Yes
Homozygous: Yes
Cell Line Description
This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.
DNA Profile
A: 101
Cw: 401
B: 3502
HLA DRB1: 11041
HLA DRB3: 202
HLA DQA1: 103
HLA DQB1: 603
HLA DPA1: 2
HLA DPB1: 201
Serological Profile
HLA A: 1
HLA A ief: 1
HLA C: 4
HLA B: 35
HLA B ief: 35.2
Bw4/bw6: 6
HLA Dw: FS
HLA DR: 11
HLA DR52: 52
HLA DQ: 6
HLA DP: 2
Culture Medium
RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.
Subculture Routine
Maintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Disclaimer
This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.
biological source: human blood. ethnicity: Ashkenasi Jewish. gender: male. growth mode: Suspension. karyotype: Not specified. morphology: Lymphoblastoid. technique(s): cell culture | mammalian: suitable. shipped in: dry ice.- UPC:
- 51442005
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 95051624-1VL