FPTase, Rat, Recombinant, E. coli (C15-1302-937)

General description

M.W. α-subunit: ~48,000; β-subunit: ~46,000

Recombinant, rat FPTase expressed in E. coli. A heterodimeric enzyme that catalyzes the transfer of a 15-carbon isoprenoid group to a C-terminal cysteine in a variety of cellular proteins, including Ras. Recognizes the CaaX motif where the farnesylated cysteine (C) is followed by two aliphatic amino acids (aa) and either leucine or serine (X). Expressed in E. coli as an untagged recombinant protein.

Recombinant, rat FPTase expressed in E. coli. A heterodimeric enzyme that catalyzes the transfer of a 15-carbon isoprenoid group to a variety of cellular proteins including Ras. Recognizes the C-terminal CAAX cysteine motif where the farnesylated cysteine is followed by two aliphatic amino acids and either leucine or serine.

Biochem/physiol Actions

1 pmol of FPTase will transfer 1 pmol of farnesyl to H-Ras in 15 min at 37°C.

Warning

Toxicity: Standard Handling (A)

Physical form

In 25 mM HEPES, pH 7.2, 40 mM NaCl, 5µM ZnCl2, and 1mM TECP

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Hightower, K.E., et al. 2001. Biochem. J.360, 625.
Zimmerman, K.K., et al. 1998. Protein Expr. Purif.14, 395.
Reiss, Y., et al. 1990. Cell62, 81.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

recombinant: expressed in E. coli. Quality Level: 200. Assay: ≥. 85% (SDS-PAGE). form: liquid. manufacturer/tradename: Calbiochem^®. . storage condition: OK to freeze, avoid repeated freeze/thaw cycles. shipped in: wet ice. storage temp.: −. 70°C. Storage Class Code: 10 - Combustible liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.