FRASER LISTERIA SUPPL. I 10 X 1 VIAL

General description

For preparation of FRASER broth, two different supplements are needed: FRASER Listeria Selective Supplement and FRASER Listeria Ammonium Iron (III) Supplement. The ammonium iron (III) citrate promotes the growth of the Listeria spp., and together with esculin (a component of the FRASER broth base), permits the β-D-glucosidase detection in Listeria spp.

Application

FRASER Listeria Ammonium iron (III) Supplement is an additive for the preparation of Half FRASER (Demi FRASER) Broth (Base) or FRASER Broth (Base) for the enrichment of Listeria spp.

Linkage

Replaces: 1000920017

Analysis Note

Growth promotion test according to ISO 11133
Mixed inoculum of Listeria, E.coli and E.faecalis
Additionaly monoculture of E.coli and E.faecalis
Pre-enrichment in Half Fraser Broth, afterwards enrichment in Fraser Broth
Confirmation on ALOA^®-Medium resp. Tryptic soy agar
Inoculum on reference medium
- Listeria monocytogenes ATCC 19111 (WDCM 00020):
10 - 100
- Listeria monocytogenes ATCC 13932 (WDCM 00021): 10 - 100
- Escherichia coli ATCC 25922 (WDCM 00013): ≥ 1000
- Escherichia coli ATCC 8739 (WDCM 00012): ≥ 1000
- Enterococcus faecalis ATCC 19433 (WDCM 00009): ≥ 1000
- Enterococcus faecalis ATCC 29212 (WDCM 00087): ≥ 1000
Blacking
- Listeria monocytogenes ATCC 19111 (WDCM 00020):
+
- Listeria monocytogenes ATCC 13932 (WDCM 00021): +
- Escherichia coli ATCC 25922 (WDCM 00013): -
- Escherichia coli ATCC 8739 (WDCM 00012): -
- Enterococcus faecalis ATCC 19433 (WDCM 00009): -
- Enterococcus faecalis ATCC 29212 (WDCM 00087): -
Growth on ALOA medium
- Listeria monocytogenes ATCC 19111 (WDCM 00020):
>10 CFU
- Listeria monocytogenes ATCC 13932 (WDCM 00021): >10 CFU
Colonies
- Listeria monocytogenes ATCC 19111 (WDCM 00020):
bluegreen with non transparent zone
- Listeria monocytogenes ATCC 13932 (WDCM 00021): bluegreen with non transparent zone
Growth on Tryptic soy agar
- Escherichia coli ATCC 25922 (WDCM 00013):
no growth
- Escherichia coli ATCC 8739 (WDCM 00012): no growth
- Enterococcus faecalis ATCC 29212 (WDCM 00087): <100 CFU
- Enterococcus faecalis ATCC 19433 (WDCM 00009): <100 CFU
Incubation:
Half Fraser Broth: 24 hours at 30°C, aerobic
Fraser Broth: 48 hours at 37°C, aerobic
Confirmation on ALOA-Medium: 48 hours at 37°C, aerobic
Confirmation on Tryptic soy agar: 24 hours at 35°C, aerobic

Legal Information

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GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany