Freund's Incomplete Adjuvant (C09-0895-639)

Only used for antibody preparation and animal immunology related research, not for human or drug use.Application：1. Freund's adjuvant can be used as an oil in water emulsion for producing immunogens. The antigens in oil-in-water emulsions can stimulate high and persistent antibody responses, which can be attributed to the slow release of antigens.2. Freund's (incomplete) adjuvant lacks the mycobacteria found in Freund's complete adjuvant, so it can minimize side effects. It is used to enhance injection. It is used to emulsify antigens injected into animal bodies, thereby producing monoclonal antibodies in the antiserum.Explanation： 1. Immunity: This product can quickly form a water in oil state when mixed with antigen. Stable lotion can be formed under the conditions of 1:1-1:2. 1:2 emulsification is thicker than 1:1 emulsification. After emulsification, water will not disperse for several weeks; The immune effect is excellent after testing multiple types of antigens and animal genera, due to the milkThoroughly and firmly solidified, it is less likely to exhibit immune tolerance compared to other adjuvants.2. Preparation of monoclonal antibody ascites: Adjuvants are injected into the abdominal cavity of BALB/C mice, with each mouse treated with 0.3-0.5ml for about 15 days (usually 12-18 days), and then hybridoma cells are injected. It is recommended to inject 800000-100000 cells per mouse. Ascites may be delayed by 1.5-2 days compared to regular adjuvants, but there is no significant differenceUnder normal circumstances, the amount of ascites is 1.5-2 times that of a regular adjuvant, and the efficacy of monoclonal antibodies in ascites remains unchanged. Related Documents: https://ald-pub-files.oss-cn-shanghai.aliyuncs.com/aladdinsci/pdp/sds/1/F393371-SCI_02a5ed42208d37864b09228ca02bc0f6.pdf