FTC-238 HUMAN THYROID CARCINOMA (C15-1314-039)

Cell Line Origin

Human follicular thyroid carcinoma, lung metastasis

Cell Line Description

FTC-238 was established from a lung metastasis of a follicular thyroid carcinoma from a 42-year-old male. The cells are polymorphic showing flat polygonal to spindle-like morphologies. Complex chromosomal changes were detected as well as a p53 mutation. FTC-133 (Sigma Catalogue number 94060901), FTC-236 (Sigma Catalogue number 06030202) and FTC-238 (Sigma Catalogue number 94060902) were all derived from the same individual, a 42 year old male with follicular thyroid cancer. This has been confirmed by short tandem repeat STR-PCR analysis at ECACC The Y chromosome could not be detected in these cell line by (STR)-PCR analysis. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell lines are identical to the source provided by the depositor based on the STR-PCR analysis.

Application

FTC-238 has been used to study the function of human thyroid and development of thyroid cancer.

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 10,11
D13S317: 11
D16S539: 11,12
D5S818: 11,12
D7S820: 9,10
THO1: 9.3
TPOX: 9
vWA: 15,18

Culture Medium

DMEM:Ham′s F12 (1:1) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:10 to 1:20 i.e. seeding at 1-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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biological source: human thyroid (lung metastasis). growth mode: Adherent. karyotype: Not specified. morphology: Polymorphic. products: Expression of 5′. - Deiodinase Type I. receptors: Not specified. technique(s): cell culture | mammalian: suitable. relevant disease(s): metastasis. shipped in: dry ice. storage temp.: −. 196°C.