Fuchs-Rosenthal Counting Chamber with 2 cover slips

The Fuchs-Rosenthal Counter is one piece construction ensuring durability and accuracy, featuring a Fuchs-Rosenthal ruling on a single plateau. TheFuchs-Rosenthal Counting Chamber is used for Cerebro-Spinal Fluid cell counts. (Cell-Depth 0.2mm) Directions for Use Usage: Cell Counts in Cerebro-Spinal Fluid Cell Depth: 0.2mm Volume: 0.2 Microliters Ruling Pattern: Fuchs-Rosenthal (Dark-Line) Diluting fluid for counting cells in Cerebro-Spinal Fluid Crystal or Methyl Violet...................................0.2 gm Glacial Acetic Acid............................................10 cc Distilled Water...................................................90 cc Collect about five CC. of spinal fluid in a sterile test tube containing a trace of Potassium Oxalate to prevent coagulation. The specimen should contain no visible trace of blood. Fill the capillary of a 1:10 pipette to the graduation with the diluting fluid and then draw the spinal fluid to the graduation above the bulb. Shake the pipette and charge the chamber as with blood corpuscle counting technique. Allow several minutes for settling. The chambers are ruled with the Fuchs-Rosenthal pattern. This consists of 16 one square millimeter areas orientated by triple lines, and each such area sub-divided into 16 squares. It is generally recommended to count 16 one square millimeter areas preferably 8 in each chamber.