Fungal Agar (Mycological Agar), 2.5 kg

Mycological media are basal media to which antifungal agents may be added for checking their effect on fungi or bacteria to render them selective for isolation and cultivation of fungi. Mycological Agar is used while working with pathogenic fungi. Earlier media for fungi generally relied on an acidic pH to make the media less suitable for the growth of many bacteria (1). Fungal Agar is prepared according to the formulation suggested by Huppert and Walker (2). Papaic digest of soyabean meal in the medium provides nitrogen, vitamins and minerals necessary to support bacterial growth. Dextrose is a carbon source required for the growth of fungi. The pH may be adjusted to 4.0 after autoclaving by adding sterile 10% lactic acid sodium (FD195)/acetic acid and used for determining yeast and mould counts of carbonated beverages and food products (3). Storage and Shelf-life: Store below 30°C in tightly closed container and prepared medium at 2-8°C. Use before expiry period on the label. References: 1. A. J. Clin. Path., 1951, 21: 684. 2. Huppert M., and Walker L. J., 1958, Am. J. Clin. Pathol., 29:291 3. Speck M. L., (Eds.), 1984, Compendium of Methods for the Microbiological Examination of Foods, 2nd Ed., APHA, Washington, D.C.