Furin Substrate, Fluorogenic - Calbiochem (C15-1302-951)

General description

A fluorogenic substrate for furin (k_cat/K_m ~2 X 10⁴ M^-1 s^-1), a mammalian homolog of the yeast Kex2 endoprotease. Also serves as a substrate for related protein, prohormone convertase 2 (PC2) (k_cat/K_m ~6 X 10³ M^-1 s^-1). Excitation max: ~360-380 nm; emission max: ~430-460 nm).

A fluorogenic substrate for furin, a mammalian homolog of the yeast Kex2 endoprotease (k_cat/K_m ~ 2 x 10⁴ s^-1M^-1).

Biochem/physiol Actions

Cell permeable: no

Primary Target
Fluorogenic substrate for furin

Product does not compete with ATP.

Reversible: no

kcat/Km ~ 2 x 104 s-1M-1 for furin

Warning

Toxicity: Standard Handling (A)

Sequence

Pyr-Arg-Thr-Lys-Arg-AMC

Physical form

Supplied as a trifluoroacetate salt.

Reconstitution

Following reconstitution, aliquot and freeze (-20°). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Lamango, N.S., et al. 1996. Arch. Biochem. Biophys.330, 238.
Hatsuzawa, K., et al. 1992. J. Biol. Chem. 267, 16094.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Molecular Weight: 827.93. Empirical Formula: C₃₇H₅₇N₁₃O₉. Quality Level: 100. Assay: ≥. 95% (HPLC). form: solid. manufacturer/tradename: Calbiochem^®. . storage condition: OK to freeze, desiccated (hygroscopic), protect from light. color: white. solubility: water: 10 . mg/mL, DMSO: soluble. fluorescence: λ. _ex . 360 - 380 . nm, λ. _em . 430-460 . nm. shipped in: ambient. storage temp.: −. 20°C. Storage Class Code: 11 - Combustible Solids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.