Cell Line Origin
Mouse Swiss-Webster myoblast
Cell Line Description
Derived from a muscle tissue culture of a foetal Swiss-Webster mouse. Tumorigenic and only slightly sensitive to acetylcholine. When confluent cultures form short multinucleated myotubes.
Application
Tumourigenicity studies
Culture Medium
DMEM + 2mM Glutamine + 10% Horse Serum (HS) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-2x10,000 cells/cm2 using 0.25% trypsin in Ca2+ + Mg2+-free PBS; 5% CO2; 37°C. Myotubes are formed at confluency, this is assisted by reducing both sera to 4%.
Other Notes
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biological source: mouse muscle. growth mode: Adherent. karyotype: Not specified. morphology: Myoblast. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 41106310
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 90110520-1VL