G-Sep SP Agarose Fast Flow, 300mL

G-Sep™ SP Agarose Fast Flow (FF) resin is a strong cation exchanger composed of highly cross-linked 6% agarose beads, with Sulphopropyl (SP) strong cation exchange groups. The resin is extensively used for biomacromolecule separations in both research and industrial applications. The G-Sep™ SP Agarose Fast Flow (FF) resins have high chemical stability, allowing well proven cleaning-in-place (CIP) and sanitization protocols and have been the industrial stand ard for ion exchange chromatography during recent decades. Other G-Sep™ Ion Exchange resins include the weak anion exhangerDEAE, theweak cation exchangerCM andthe strong anion exhangerQ . Features Matrix: Cross-linked agarose beads, 6% Ligand : Sulphopropyl Ion Exchanger : Strong cation exchanger Bead form: Spherical, diameter 45-165μm Ionic Capacity: 0.18-0.25 mmol (Na + )/ml Binding Capacity: 70 mg lysozyme /ml medium pH stability Working Range: 2-12 pH stability Cleaning-in-Place (CIP): 2-14 Maximum Flow Velocity: 450cm/h Maximum Pressure: 0.3MPa Exclusion limit(globular proteins): 4 x 10 6 Physical Stability: Negligible volume variation due to changes in pH or ionic strength Chemical Stability: Stable to all commonly used aqueous buffers:1 M NaOH, 8M urea, 8M guanidine hydrochloride, 70% ethanol Autoclavable: With Na + as counter Ions, at 121°C, pH 7, for 30 min in 0.2M sodium acetate for autoclaving Storage Conditions: 4 to 30°C, 20% Ethanol containing 0.2M sodium acetate Applications Ion exchange chromatography