G-Trap HIC Kit, 4 x 1mL

G-Trap™ HIC Selection Kit consists of 4 prepacked 1 ml columns (G-Trap™ HIC Columns) with matrix or resin for hydrophobic interaction chromatography. The kit consists of 4 pepacked columns namely G-Trap™ Butyl Agarose 6 Fast Flow, G-Trap™ Octyl Agarose 6 Fast Flow, G-Trap™ Phenyl Agarose 6 Fast Flow (High Sub) and G-Trap™ Phenyl Agarose 6 Fast Flow (Low Sub). G-Trap™ HIC Selection Kit allows for initial screening of appropriate ligand /matrix and conditions for isolation of a specific protein or biomolecule. Once appropriate resin/medium is determined, individual G-Trap™ HIC Column in 1 ml or 5 ml sizes can be used. Individual G-Trap™ HIC Columns are available at G-biosciences in 1 ml and 5 ml column volume size The G-Trap™ HIC Columns available for seperataion of the target protein includes G-Trap™ Butyl Agarose 6 Fast Flow , G-Trap™ Octyl Agarose 6 Fast Flow , G-Trap™ Phenyl Agarose 6 Fast Flow (High Sub) and G-Trap™ Phenyl Agarose 6 Fast Flow (Low Sub). Biomolecules are separated on the HIC column based upon the hydrophobicity of the ligand attached to the resin, the distribution of surface-exposed hydrophobic amino acids present on the protein or enzyme to be separated and the concentration and type of salt used in binding or start buffer. Straight alkyl chains ligand s such as butyl, octyl show pure hydrophobic character whereas aryl ligand s such as phenyl show mixed mode behavior where aromatic, hydrophobic as well as lack of charge play role in protein/biomolecules adsorption to the column. In HIC, the binding of biomolecules to the column (ligand ) is facilitated by using higher concentrations of anti-chaotropic salts such as ammonium sulfate, sodium sulphate etc in the binding buffer. The properties ofG-Trap™ HIC Selection Kit resin and column are listed below G-Trap™ HIC Selection Kit resin G-Trap™ Butyl Agarose 6 Fast Flow Resin Mean particle size : 90 µm Bead structure : Highly cross-linked 6% Agarose Ligand : Butyl Ligand Concentration : About 40μmol/ml Binding Capacity : About 20mg HSA/ml resin pH stability Working Range : 3-13 pH stability Cleaning-in-Place (CIP) : 2-14 Maximum Pressure (MPa): 0.3 Maximum Flow Velocity : 450cm/h Exclusion Limit (Globular Proteins) : 4 x 106 Physical Stability : Negligible volume variation due to changes in pH or ionic strength Chemical Stability: Stable to all commonly used aqueous buffers:1 M NaOH, 8 M urea, 8 M guanidine hydrochloride, 70% ethanol Storage Conditions : 4 to 30°C, 20% Ethanol G-Trap™ Octyl Agarose 6 Fast Flow Resin Mean particle size : 90 µm Bead structure : Highly cross-linked 6% Agarose Ligand : Octyl Ligand Concentration : About 5μmol/ml Binding Capacity : About 30mg HSA/ml resin pH stability Working Range : 3-13 pH stability Cleaning-in-Place (CIP) : 2-14 Maximum Pressure (MPa) : 0.3 Maximum Flow Velocity : 450cm/h Exclusion Limit (Globular Proteins) : 4 x 106 Physical Stability : Negligible volume variation due to changes in pH or ionic strength Chemical Stability: Stable to all commonly used aqueous buffers:1 M NaOH, 8 M urea, 8 M guanidine hydrochloride, 70% ethanol Storage Conditions : 4 to 30°C, 20% Ethanol G-Trap™ Phenyl Agarose 6 Fast Flow (High Sub) Resin Mean particle size : 90 µm Bead structure : Highly cross-linked 6% Agarose Ligand : Phenyl Ligand Concentration : About 40μmol/ml Binding Capacity : About 40mg HSA/ml resin pH stability Working Range : 3-13 pH stability Cleaning-in-Place (CIP) : 2-14 Maximum Pressure (MPa) : 0.3 Maximum Flow Velocity : 450cm/h Exclusion Limit (Globular Proteins) : 4 x 106 Physical Stability : Negligible volume variation due to changes in pH or ionic strength Chemical Stability : Stable to all commonly used aqueous buffers:1 M NaOH, 8 M urea, 8 M guanidine hydrochloride, 70% ethanol Storage Conditions : 4 to 30°C, 20% Ethanol G-Trap™ Phenyl Agarose 6 Fast Flow (Low Sub) Resin Mean particle size : 90 µm Bead structure : Highly cross-linked 6% Agarose Ligand : Phenyl Ligand Concentration : About 25 μmol/ml Binding Capacity: About 20 mg HSA/ml resin pH stability Working Range : 3-13 pH stability Cleaning-in-Place (CIP) : 2-14 Maximum Pressure (MPa) : 0.3 Maximum Flow Velocity : 450 cm/h Exclusion Limit (Globular Proteins) : 4 x 106 Physical Stability : Negligible volume variation due to changes in pH or ionic strength Chemical Stability: Stable to all commonly used aqueous buffers:1 M NaOH, 8 M urea, 8 M guanidine hydrochloride, 70% ethanol Storage Conditions: 4 to 30°C, 20% Ethanol G-Trap™ HIC Selection Kit, 1 ml column Column Volume : 1 ml Columns Dimension : 0.7 x 2.5 cm Column Harware Pressure Limit : 0.5 MPa Column Hardware : Polypropylene (Biocompatible) Application(s): G-Trap™ HIC Selection Kit allows for initial screening of appropriate ligand /matrix and conditions for isolation of a specific protein or biomolecule.The kit consists of 4 pepacked columns namely G-Trap™ Butyl Agarose 6 Fast Flow, G-Trap™ Octyl Agarose 6 Fast Flow, G-Trap™ Phenyl Agarose 6 Fast Flow (High Sub) and G-Trap™ Phenyl Agarose 6 Fast Flow (Low Sub).