G6P-DH GRADE I

General description

Glucose-6-phosphate dehydrogenase (G6P DH) has an active dimeric form, whereas its monomeric and tetrameric forms are inactive. At low NADP⁺ and NADPH levels and under normal conditions, the enzyme exists as equilibrium of tetramers and dimers, whereas at high NADP⁺ and NADPH levels the equilibrium shifts to inactive tetramers.

Application

Component of cofactor recycling systems for NADPH.

Biochem/physiol Actions

Glucose-6-phosphate dehydrogenase (G6P-DH) is involved in the pentose phosphate pathway. It is responsible for catalysing the oxidation of glucose-6-phosphate (G6P) to gluconolactone-6-phosphate, with NADP⁺ simultaneously accepting hydrogen to form NADPH, thereby maintaining NADPH levels in cells.

Specifications

Contaminants: <0.001% CK, <0.01% GR, HK, 6-PGDH, and PGluM each, <0.002% PGI each, <0.002% PGI

Approximately 350U/mg at +25°C with glucose-6-phosphate as the substrate.

Physical form

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6

Preparation Note

Activator: Mg²⁺ (5 to 10 mM)

Other Notes

For life science research only. Not for use in diagnostic procedures.