Application
Lee, et al., reported a non-radioactive version of in vitro phosphorylation where γ-[(6-Azidohexyl)-imido]-ATP (compound 6) was successfully used instead of γ-32P-modified ATP to phosphorylate GST-tagged recombinant p27kip1 with protein kinase cdk2. The phosphorylated, azide-modified protein substrate can be labeled with alkynes of biotin or fluorescent dyes using Cu(I)-catalyzed click chemistry or DBCO-containing biotin or fluorescent dyes using Cu(I)-free click chemistry.
Molecular Weight: 631.37 (free acid basis). Empirical Formula: C16H28N9O12P3 · xNa+. Assay: ≥. 95% (HPLC). Quality Level: 200. form: powder. reaction suitability: reaction type: click chemistry. solubility: 10 mM Tris-HCl, pH 7.5: soluble. shipped in: wet ice. storage temp.: −. 20°C. SMILES string: O[C@@H]([C@H]1O)[C@@H](COP(O)(OP(O)(OP(NCCCCCCN: [N+]: [N-])(O): O): O): O)O[C@@H]1N2C: NC3: C2N: CN: C3N. InChI: 1S/C16H28N9O12P3/c17-14-11-15(20-8-19-14)25(9-21-11)16-13(27)12(26)10(35-16)7-34-39(30,31)37-40(32,33)36-38(28,29)23-6-4-2-1-3-5-22-24-18/h8-10,12-13,16,26-27H,1-7H2,(H,30,31)(H,32,33)(H2,17,19,20)(H2,23,28,29)/t10-,12-,13-,16?/m1/s1. InChI key: FZLLGYSKHRTLRX-AARXTDBFSA-N. Storage Class Code: 11 - Combustible Solids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable.- UPC:
- 51141566
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 808520-1MG