Application
Lee, et al,. reported a non-radioactive version of in vitro phosphorylation where γ-[(Propargyl)-imido]-ATP (compound 1) was successfully used instead of γ-32P-modified ATP to phosphorylate GST-tagged recombinant p27kip1 with protein kinase cdk2. The phosphorylated, alkyne-modified protein substrate can be labeled with azides of biotin or fluorescent dyes using Cu(I)-catalyzed click chemistry.
Molecular Weight: 544.24 (free acid basis). Empirical Formula: C13H19N6O12P3 · xNa+. Assay: ≥. 95% (HPLC). Quality Level: 200. form: solid. reaction suitability: reaction type: click chemistry. solubility: 10 mM Tris-HCl, pH 7.5: soluble. shipped in: wet ice. storage temp.: −. 20°C. SMILES string: NC1: NC: NC2: C1N: CN2[C@H]3O[C@H](COP(OP(O)(OP(O)(NCC#C): O): O)(O): O)[C@@H](O)[C@H]3O. InChI: 1S/C13H19N6O12P3/c1-2-3-18-32(22,23)30-34(26,27)31-33(24,25)28-4-7-9(20)10(21)13(29-7)19-6-17-8-11(14)15-5-16-12(8)19/h1,5-7,9-10,13,20-21H,3-4H2,(H,24,25)(H,26,27)(H2,14,15,16)(H2,18,22,23)/t7-,9-,10-,13?/m1/s1. InChI key: PIPOEUYSJJTELD-RJNFYWFKSA-N. Storage Class Code: 11 - Combustible Solids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable.- UPC:
- 51141566
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 808512-1MG