General description
An internally quenched fluorogenic peptide substrate containing the C-terminal β-APP (amyloid β-peptide precursor protein) amino acid sequence that is cleaved by γ-secretase. Shown to be sensitive and useful for assaying γ-secretase activity. The proteolysis at the Aβ40-, Aβ42-, and Aβ43-generating cleavage sites results in enhanced fluorescence.
An internally quenched fluorogenic peptide substrate containing the C-terminal β-APP (amyloid β-peptide precursor protein) amino acid sequence that is cleaved by γ-secretase. Shown to be sensitive and useful for assaying γ-secretase activity. The proteolysis at the Aβ40-, Aβ42-, and Aβ43-generating cleavage sites results in enhanced fluorescence. Excitation max.: ~355 nm; emission max.: ~440 nm.
Biochem/physiol Actions
Cell permeable: no
Product does not compete with ATP.
Reversible: no
Packaging
Packaged under inert gas
Warning
Toxicity: Standard Handling (A)
Sequence
NMA-Gly-Gly-Val-Val-Ile-Ala-Thr-Val-Lys(DNP)-D-Arg-D-Arg-D-Arg-NH₂
Reconstitution
Following reconstitution, aliquot, flush with a stream of inert gas, and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Other Notes
Farmery, M.R., et al. 2003. J. Biol. Chem.278, 24277.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Molecular Weight: 1609.83. Empirical Formula: C70H116N26O18. Quality Level: 100. Assay: ≥. 95% (HPLC). form: lyophilized. manufacturer/tradename: Calbiochem®. . storage condition: OK to freeze, desiccated (hygroscopic), protect from light. color: yellow. solubility: DMSO: 5 . mg/mL, 5% acetic acid: soluble. shipped in: ambient. storage temp.: −. 20°C. Storage Class Code: 11 - Combustible Solids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.- UPC:
- 41116127
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 565764-1MG