General description
Recombinant, mouse growth differentiation factor-8 (GDF-8)/myostatin propeptide, consisting of amino acids 25-285, fused at the C-terminus to a His•Tag
Application
GDF-8 Propeptide, His•Tag
Biochem/physiol Actions
ED₅₀ = 0.05-0.25 µg/ml (using 40 ng/ml rmGDF-8) as measured by inhibition of rmGDF-8 activity in K562 human chronic myelogenous leukemia cells. Approximately 1 µg/ml will completely inhibit GDF-8 activity in K562 cells.
Warning
Toxicity: Standard Handling (A)
Physical form
Lyophilized from 0.2 µm filtered PBS, BSA.
Reconstitution
Following reconstituyion refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Reconstitute to 50 µg/ml with sterile PBS containing ≥1% human or bovine serum albumin.
Other Notes
Hill, J.J. et al. 2003. Molecular Endocrinology17, 1144.
Thies, R.S. et al. 2001. Growth Factors18, 251.
Chang, H. et al. 2002. Endocrine Reviews23, 787.
McPherron, A.C. et al. 1997. Nature387, 83.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
recombinant: expressed in mouse NSO cells (myeloma cells). Quality Level: 100. Assay: ≥. 90% (SDS-PAGE). form: lyophilized. manufacturer/tradename: Calbiochem®. . storage condition: OK to freeze. impurities: ≤. 1.0 . EU/μ. g protein Endotoxin. shipped in: ambient. storage temp.: −. 20°C. Storage Class Code: 11 - Combustible Solids. WGK: WGK 2.- UPC:
- 12352200
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 345749-25UG