GenCrispr Mutation Detection Kit,25reactions

The GenCrispr Mutation Detection Kit provides a simple, reliable, and rapid method for the detection of site specific cleavage of genomic DNA that is extracted from cells transfected with constructs expressing engineered nucleases such as Transcription activator-like effector nucleases (TALEN), Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9, or Zinc-finger nucleases (ZFN). The GenCrispr Mutation Detection Kit includes High-Fidelity DNA polymerase for amplifying the target regions from cells, and GenCrispr T7 Endonuclease I for recognizing and detecting the mismatches caused by gene editing tools. It provides an easy and reliable approach for estimating the efficiency of genome editing.   Components Amount provided 25-reaction kit 100-reaction kit High-Fidelity DNA polymerase 10 μL 40 μL 5X PCR Reaction Buffer 150 μL 600 μL GenCrispr T7 Endonuclease I ( Cat.No.Z03396 ) 25 μL 100 μL 10X GenCrispr T7 Endonuclease I Reaction buffer 50 μL 500 μL Control Template DNA 30 μL 120 μL Control Primer Mix 30 μL 120 μL Protease K 25 μL 100 μL dNTP 15 μL 60 μL