Cas9 Nuclease NLS, S. pyogenes , is an RNA-guided endonuclease that catalyzes site-specific cleavage of double stranded DNA. The location of the break is within the target sequence 3 bases from the NGG PAM (Protospacer Adjacent Motif). The design of single guide RNA (sgRNA) is dependent on the target region close to the PAM site. Even if you pick a target sequence that fulfills all of the described requirements, sgRNA specificity and activity is unpredictable. Therefore, it is often recommended that multiple, different sgRNAs be designed to target a gene of interest. The GenCrispr sgRNA Screening Kit provides a simple, reliable, and rapid method for assessing sgRNA efficiency before cell transduction, allowing you to identify the highly effective CRISPR sgRNA. Components Amount 30-reaction kit 100-reaction kit GenCrispr Cas9 Nuclease 15 μL 50 μL 10X Reaction Buffer 100 μL 300 μL Positive Control sgRNA 3 μg 10 μg Positive Control Substrate 60 μL 200 μL RNase-free water 1 mL 1 mL
- UPC:
- 41122409
- Condition:
- New
- Availability:
- 3 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- L00689-30
