GenCRISPRT SaCas9 2NLS Nuclease, 0.1mg

The GenCRISPR™ SaCas9 2NLS Nuclease can be formed with the guide RNA into a ribonucleoprotien (RNP) complex. The use of an RNP complex to perform gene editing has been shown to reduce the challenges encountered with other CRISPR gene editing techniques such as viral and plasmid delivery. Challenges include off-target effects, cell viability and transcription/translational challenges. The SaCas9 recognizes an NNGRRT protospacer adjacent motif (PAM) and cleaves target DNA at high efficiency with a variety of guide RNA (gRNA) spacer lengths. GenCRISPR™ SaCas9 2NLS Nuclease is a tag free nuclease produced by expression in an E. coli strain carrying a plasmid encoding the Cas9 gene from Staphylococcus aureus with a nuclear localization signal at both N-terminal and C-terminal.The small size of the nuclease facilitates enhanced in vivo delivery for genome editing in various organisms. For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.