Genomiphi(TM) V2 DNA Amplification Kit (C15-1426-794)

General description

Illustra GenomiPhi^™ V2 DNA Amplification Kit offers highly efficient and representative whole-genome amplification with 4 to 7 μg yield from nanogram amounts of DNA sample.

illustra^™ GenomiPhi^™ V2 DNA Amplification Kit is part of the Phi29 DNA polymerase family of products from Cytiva. It contains all of the components necessary for mini-scale whole genome amplification by isothermal strand displacement amplification.

Amplification is highly uniform over the entire genome so that locus representation remains extremely close to the original DNA sample. Furthermore, amplification is carried out with very high fidelity due to Phi29 DNA polymerase proofreading activity.

The illustra^™ GenomiPhi^™ V2 DNA Amplification Kit procedure is quick, simple and does not require a thermocycler. A typical DNA yield of 4 to 7 μg DNA can be achieved in less than two hours with little hands-on time. The average product length is over 10 kb. The starting material for GenomiPhi^™ reactions can be purified DNA from any commercial kit or homebrew method, or a nonpurified cell lysate may be used. The kit was verified with DNA from various clinical samples including FTA^™ card lysates, blood and buccal swabs.

Application

GenomiPhi^™ amplified DNA is suitable for various applications such as genotyping (SNP, STR, array CGH), cloning, sequencing, and DNA archival.

Features and Benefits

• Representative isothermal amplification of the whole genome.
• No template-independent, background amplification product.
• Outperforms PCR-based whole genome amplification techniques.
• Quick and simple automation-friendly protocol; no thermal cycler required.
• Yields high-quality DNA (e.g., for high-throughput genotyping, hybridization, and DNA archival).

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

GenomiPhi
On packaging and insert:
For use only as licensed by Qiagen GmbH. and Cytiva. The Phi 29 DNA polymerase may not be re-sold or used except in conjunction with the other components of this kit. See US patent numbers 5,854,033, 6,124,120, 6,143,495, 6,323,009, 5,576,204, and equivalent patents and patent applications in other countries.
On container:
Subject to proprietary rights of Cytiva. May not be re-sold or used except with the other components of this kit. See US patent numbers 5,576,204, and equivalent patents and patent applications in other countries.

FTA is a trademark of Qiagen Group

GenomiPhi is a trademark of Cytiva

illustra is a trademark of Cytiva