Cell Line Origin
Rat Wistar-Furth pituitary tumour
Cell Line Description
Derived from a 7 month female Wistar-Furth rat. GH3 cells produce growth hormone faster than the GH1 cell line and also produce prolactin. Hydrocortisone stimulates growth hormone production and inhibits prolactin production. Studies have shown that cells are capable of adaptation to suspension culture using MEM.
Application
Hormone studies
Culture Medium
Ham′s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Upon thawing, the time taken before the first subculture can be performed may be extended (10-15 days). To aid attatchment, flask
Other Notes
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biological source: rat brain. growth mode: Adherent. karyotype: Hyperdiploid, modal no. 60. morphology: Epithelial. products: Growth hormone, prolactin. receptors: Not specified. technique(s): cell culture | mammalian: suitable. relevant disease(s): cancer. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 51183000
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 87012603-1VL
