General description
Molecular Weight: 160 kDa (gel filtration)
pI: 4.2
Extinction coefficient: E1% = 16.7 (280 nm)
Glucose oxidase from Aspergillus niger is a dimer consisting of 2 equal subunits with a molecular mass of 80 kDa each. Each subunit contains one flavin adenine dinulceotide moiety and one iron. The enzyme is a glycoprotein containing ~16% neutral sugar and 2% amino sugars. The enzyme also contains 3 cysteine residues and 8 potential sites for N-linked glycosylation.
Glucose oxidase is capable of oxidizing
The pH optimum for glucose oxidase is 5.5, while it has a broad activity range of pH 4-7. Glucose oxidase is specific for β-
Glucose oxidase does not require any activators, but it is inhibited by Ag+, Hg2+, Cu2+, phenylmercuric acetate, and p-chloromercuribenzoate. It is not inhibited by the nonmetallic SH reagents: N-ethylmaleimide, iodoacetate, and iodoacetamide.
Glucose oxidase can be utilized in the enzymatic determination of
Application
Several publications cite use of the G2133 glucose oxidase in their protocols and in various applications, such as the following:
a) Biosensor development:
- Diazoresin nanofilm coatings on alginate microspheres: Srivastava, R. et al., Biotechnol. Bioeng., 91(1), 124-131 (2005).
- Paper-based glucose biosensor: Lankelma, J. et al., Anal. Chem., 84(9), 417-4152 (2012)
- Microfluidic device with glucose oxidase immobilized on hydrogel for glucose analysis of blood: He, R.-Y. et al., RSC Adv., 9, 32367-32374 (2019).
c) Enzymatic fuel-cells with chitosan-based membranes: Bahar, T., and Yazici, M.S., Electroanalysis, 32(6), 1304-1314 (2020).
Glucose oxidase is widely used in the food and pharmaceutical industries as well as a major component of glucose biosensors.
Biochem/physiol Actions
Glucose oxidase catalyses the oxidation of β-d-glucose to d-glucono-β-lactone and hydrogen peroxide, with molecular oxygen as an electron acceptor.
Quality
May contain traces of amylase, maltase, glycogenase, invertase, and galactose oxidase.
Unit Definition
One unit will oxidize 1.0 μmole of β-
Physical form
Lyophilized powder containing phosphate buffer salts and sodium chloride
Analysis Note
Protein determined by biuret.
- UPC:
- 41202004
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- G2133-10KU