General description
Glutamic-Oxalacetic Transaminase is found in all tissues and is predominant in the liver and skeletal muscle. It is a prototype of fold-type I pyridoxal 5′-phosphate (PLP)-enzymes. Glutamic-oxalacetic transaminase is a homodimer containing large and small domains in each subunit.
Application
Glutamic-Oxalacetic Transaminase from porcine heart has been used:
- in an enzyme-coupled assay for evaluating
L -asparaginase enzyme activity - in microtiter plate format enzyme-based assay to estimate malic acid content in berry samples
- as a supplement in homogenization buffers for the isolation of heart mitochondrial membranes in the presence of oxaloacetate (OAA)-depleting system
Biochem/physiol Actions
Glutamic-oxalacetic transaminase or aspartate aminotransferase (AAT) catalyzes the interconversion of
Unit Definition
One unit will convert 1.0 μmole of α-ketoglutarate to
Physical form
Suspension in 3.0 M (NH4)2SO4 containing 0.05 M maleate and 2.5 mM α-ketoglutarate, pH 6.0
Analysis Note
Protein determined by biuret.
- UPC:
- 51184012
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- G2751-1KU