General description
Glutaraldehyde (Glutaric dialdehyde, Pentane-1,5-dial) is an aliphatic dialdehyde that can be employed as an effective biochemical reagent for various purposes such as a protein crosslinker, enzyme immobilization in microscopy, histochemistry, and cytochemistry. It serves as an amine-reactive homobifunctional crosslinker and a cell fixative before SDS-PAGE, staining, or electron microscopy, making it suitable for a wide range of morphological studies.
Glutaraldehyde is capable of efficiently crosslinking amine and hydrazine derivatives to proteins and other amine-containing polymers. Notably, in certain reactions, biotin hydrazides have been directly coupled to nucleic acids using glutaraldehyde, presenting a potential utility in conjugating fluorescent hydrazides and hydroxylamines to DNA. In histology studies, Glutaraldehyde is also employed to preserve tissue sections and prepare them for closer examination.
Application
Cross-linking agent for gelatin, poly(vinyl alcohol), and polyheptapeptides.
Glutaraldehyde may be used in the following studies:
- To compose the fixative solution (Glutaraldehyde + Paraformaldehyde + NaPO4) for use in high-resolution light microscopy and electron microscopy studies.
- To study the conjugation of goat anti-horseradish peroxidase with alkaline phosphatase by a reported method.
- To compose the primary fixative, which is employed to protect the deterioration of cytoplasmic features of yeast cells during permanganate fixation.
Features and Benefits
- Versatile and adaptable for wide variety of research applications
- Ready-made solution reduces the need for preparation time
Other Notes
To gain a comprehensive understanding of our extensive range of Monosaccharides for your research, we encourage you to visit our Carbohydrates Category page.
- UPC:
- 12352500
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 340855-25ML