General description
Native glutaredoxin-S2 isolated from E. coli. Glutaredoxin functions as a glutathione-dependent hydrogen donor for ribonucleotide reductase. It is useful as a general disulfide reductant for the in vitro study of protein folding mechanisms and has been demonstrated to work in conjunction with protein disulfide isomerase to enhance refolding of scrambled RNase A and RNase T1.
Warning
Toxicity: Standard Handling (A)
Unit Definition
Units are defined using a standard HED assay.
Physical form
Lyophilized from 0.5% NH₄HCO₃.
Reconstitution
Reconstitute in 1 ml 50 mM Tris-HCl, 1 mM EDTA, pH 7.5 to yield a final concentration of 1 mg/ml.
Other Notes
Ruoppolo, M., et al. 1997. Biochemistry36, 12259.
Sun, C., et al. 1997. Protein Sci.6, 383.
Prinz, W.A., et al. 1997. J. Biol. Chem.272, 15661.
Holmgren, A. and Alund, F. 1995 Methods Enzymol.252, 283.
Hoog, J.O., et al. 1986. Gene43, 13.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Quality Level: 100. form: lyophilized. specific activity: ≥. 175 . μ. mole/min-mg (NADPH oxidized). manufacturer/tradename: Calbiochem®. . storage condition: OK to freeze. shipped in: wet ice. storage temp.: 2-8°C. Storage Class Code: 11 - Combustible Solids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.- UPC:
- 12352204
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 354406-1MG
