GLUTATHIONE ASSAY, 450 ASSAYS

Reduced glutathione (L-γ-glutamyl-L-cysteinylglycine), a tripeptide, serves as a key antioxidant in animal, plant, fungi and bacteria by providing free thiol. Glutathione exist in reduced (GSH) and oxidized (GSSG; gluthathione disulphide) forms in cells and tissues, and the concentration of glutathione range from 0.5 to 10mM in animal cells 1 . The majority (90-95 %) of glutathione exist in reduced form (GSH) in healthy cells. GSH provides reducing equivalents to antioxidant enzymes, hydroxyl radicals, ROS and is itself oxidized to GSSG. Therefore GSH/GSSG ratio is critical indicator of the health of cell. During oxidative stress there is decrease in levels of GSH and increase in levels of GSSG and thus GSH/GSSG ratio decreases. Glutathione colorimetric assay kit is designed to measure reduced glutathione (GSH), oxidized glutathione (GSSG) and total glutathione (GSH+GSSG) concentrations in wide range of samples like, blood, plasma, serum, cultured cells and tissue. The assay involves carefully optimized enzymatic recycling method using glutathione reductase and Ellman’s reagent (DTNB) 2 . Features Measure total, oxidized (GSSG) and reduced (GSH) glutathione colorimetrically Assay Range:0.1-2.5 μM for GSSG and for GSH it would be 0.2-5 μM Plated based, colorimetric measurement at 405-415nm End-point and kinetic methods 225 and 450 assay sizes available The assay is designed to measure glutathione in wide range of samples such as plasma, blood, serum, urine, cultured cells, tissue etc. Applications Total Glutathione Colorimetric assay kit measures total, oxidized and reduced glutathione in wide range of samples such as blood, plasma, serum, cultured cells and tissues Estimating glutathione in cells is one of the ways to measure oxidative stress and this assay is also vital for mitochondrial toxicity screening GSH/GSSG ratio decreases in many pathological diseases such as cancer, diabetes, cardiovascular disorders, artherosclerosis, Parkinson’s and Alzheimer’s 3, 4 and thus estimating glutathione is vital in this area of research.