GLYCINE FOR ELECTROPHORESIS (C005B-049061)

General description

Glycine is a molecule with pI of 6.7, which is similar to the pH of stacking gel. It has advantage of low mobility, hydrophobicity and it does not associate with proteins. Glycine is a component of Tris-glycine and Tris-glycine-SDS (sodium dodecyl sulfate) running buffers for polyacrylamide gel electrophoresis. Glycine is also a component of Towbin′s transfer buffer for Western blots.

Glycine, an achiral proteinogenic amino acid, serves diverse roles in biochemistry and physiology. As a non-essential, non-polar glucogenic amino acid, it acts as an inhibitory neurotransmitter in the central nervous system and co-agonist at NMDA receptors with glutamate. Serving as a precursor, glycine is a crucial building block for macromolecules, playing a vital role in cellular processes.

In addition to its biological functions, glycine exhibits versatility in research applications. Its role as a biochemical reagent makes it valuable in assays and procedures, while its buffering capacity is essential in techniques like SDS-PAGE, Western Blotting, chromatography, and cell culture. Glycine′s zwitterionic nature makes it an effective buffer across various pH values, widely used in immunology research for buffer preparation in techniques such as Western Blotting. Compatible with enzymes, glycine is useful in enzymatic assays like lactate determination. Moreover, glycine contributes to buffer formulations for protein stabilization, pH control, and enzymatic reactions.

Application

• Glycine has been used for the preparation of gelatin composites scaffold.
• It has been used in the elution buffer to extract protein using HiTrap protein G column.
• It has been used in the SDS-sample, running and transfer buffers prepared for SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) and western blotting.

Biochem/physiol Actions

Inhibitory neurotransmitter in spinal cord, allosteric regulator of NMDA receptors.

Features and Benefits

• Suitable for Cell Biology and Biochemical studies
• High-quality compound suitable for multiple research applications

Analysis Note

Tested for use in electrode buffers for PAGE and in transfer buffers for Western blots.