Goat Affinity Purified Antibody To Hamster IgG (whole Molecu

Analysis Note The total protein is measured by absorbance (A280nm). Antibody titer is standardized by equivalence-point precipitation. Each affinity purified antibody is tested for purity at 10 mg/mL using immunoelectrophoresis. The product is goat IgG; no trace of albumin is detected. Preparation Method The affinity purified antibody is prepared from the specific goat IgG fraction by solid phase absorption. The resulting affinity purified antibody is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, adjusted to standard titer, filtered through a 0.22 µm filter, and vialed. No preservative has been added. Applications Immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections. Product Description Product is goat affinity purified antibody to hamster IgG (whole molecule) and buffer salts. Typical Working Concentration Suggested working dilutions: 1:50-1:200 for Tissue Staining; 1:100 - 1:500 for Immunoblotting; 1:400 - 2,000 for Elisa