Goat Affinity Purified Antibody to Rat Igg (Whole Molecule),

Goat affinity purified antibody to rat igg (whole molecule) is goat affinity purified antibody to rat IgG (whole molecule) and buffer salts. Analysis Note The total protein is measured by absorbance (A280 nm). Antibody titer is standardized by equivalence-point precipitation. Each affinity purified antibody is tested for purity at 10 mg/mL using immunoelectrophoresis. The product is goat IgG; no trace of albumin is detected. Preparation Method The affinity purified antibody is prepared from the specific goat IgG fraction by solid phase absorption. The resulting affinity purified antibody is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, adjusted to standard titer, filtered through a 0.22 µm filter, and vialed. No preservative has been added. Background Information Anti-Rat IgG whole molecule antibody generated in goat detects specifically Rat IgG whole molecule. This secondary antibody anti-Rat is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays. Applications Goat affinity purified antibody to rat igg (whole molecule) is used in immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections. This product is suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. This product is suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers, and for attachment to solid supports for use as an immunoadsorbant. This product is suitable for use as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or tissue immunostaining. These affinity purified antibodies are recommended to avoid non-specific binding from the inherent antibodies of host animals. Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors.