Goat Anti-Human IgA Antibody, IgG, and IgM, HRP conjugate (C15-1319-102)

General description

IgG is the most abundant immunoglobulin comprising two heavy chains and two light chains. Each molecule has two antigen binding sites. IgG has 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%). IgM constitutes about 10% of serum immunoglobulins. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells. Monomeric IgA constitutes 5-15% of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces.

Specificity

The antibody reacts with human IgG, IgM and IgA. May also react with the light chains of other human immunogloibulins.

Immunogen

Prepared from purified human IgG, IgM and IgA.

Application

Detect Human IgA using this Goat anti-Human IgA Antibody, IgG & IgM, HRP conjugate validated for use in ELISA, WB, IH.

EIA: 1:5,000-1:100,000.
Western blots: 1:5,000-1:200,000.
Immunohistochemistry: 1:500-1:5,000.

Optimal working dilutions must be determined by the end user.

Research Category
Secondary & Control Antibodies

Research Sub Category
Whole Immunoglobulin Secondary Antibodies

Physical form

ImmunoAffinity Purified

Purified by immunoaffinity chromatography using antigen coupled to agarose beads. Lyophilized. Buffer is 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA. Contains no preservative.

Storage and Stability

Maintain lyophilized product at 2°-8°C for up to 12 months from date of receipt. After reconstitution the product is stable for six weeks at 2°-8°C as an undiluted liquid. Reconstitute vial with 2 mL of distilled water. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of antibody (and buffer salts) will decrease to one-half of the original after the addition of glycerol and final working dilutions must be adjusted accordingly. Avoid repeated freeze thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: goat. Quality Level: 100. conjugate: peroxidase conjugate. antibody form: affinity purified immunoglobulin. antibody product type: secondary antibodies. clone: polyclonal. species reactivity: human. manufacturer/tradename: Chemicon^®. . technique(s): ELISA: suitable, immunohistochemistry: suitable, western blot: suitable. shipped in: wet ice. target post-translational modification: unmodified. Storage Class Code: 11 - Combustible Solids. WGK: WGK 3.