General description
IgM constitutes about 10% of serum immunoglobulins. IgM is prominent in early immune responses to most antigens and predominates in certain antibody responses such as ′natural′ blood group antibodies. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells.
Specificity
Based on immunoelectrophoresis, the antibody reacts with the Fc5μ portion of the human IgM heavy chain. Does not react with human IgG or IgA, or with the light chains of human immunoglobulins. No reactivity against non-immunoglobulin serum proteins, but the antibody may cross-react with IgM from other species.
Immunogen
Prepared from purified human IgM Fc5μ fragment.
Application
EIA: 1:5,000-1:100,000.
Western blots: 1:5,000-1:200,000.
Immunohistochemistry: 1:500-1:5,000.
Optimal working dilutions must be determined by the end user.
Goat anti-Human IgM Antibody, Fc5μ, HRP conjugate is an antibody against Human IgM for use in ELISA & WB.
Research Category
Secondary & Control Antibodies
Research Sub Category
Fragment Specific Secondary Antibodies
Physical form
0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 with 15mg/mL BSA. Contains no preservative. Lyophilized.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
biological source: goat. Quality Level: 100. conjugate: peroxidase conjugate. antibody form: affinity isolated antibody. antibody product type: secondary antibodies. clone: polyclonal. species reactivity: human. manufacturer/tradename: Chemicon®. . technique(s): ELISA: suitable, western blot: suitable. shipped in: wet ice. target post-translational modification: unmodified. Storage Class Code: 13 - Non Combustible Solids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable.- UPC:
- 12352207
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- AP114P