General description
Immunoaffinity purified goat polyclonal antibody conjugated to β-galactosidase. Recognizes mouse IgG, heavy and light chains.
This Goat Anti-Mouse IgG, H & L Chain β-Galactosidase Conjugate is validated for use in Enzyme Immunoassay, Immunoelectrophoresis for the detection of Mouse IgG, H & L Chain.
Application
Enzyme Immunoassay (1:1000)
Immunoelectrophoresis (see comments)
Packaging
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
Physical form
In 50 mM PBS, 10 mM glycine, 10 mM MgCl₂, 1% BSA, pH 7.0.
Other Notes
To achieve maximum enzyme activity, we recommend using the following substrate:
Dissolve 1 mg 4-methylumbelliferyl-β-D-galactoside (Cat. No. 474424) in 10 ml substrate buffer (10 mM sodium phosphate, pH 7.8, with 1 mM MgCl2) by stirring for 30 min at room temperature. Remove undissolved substrate by centrifuging or filtration.
Monospecific for mouse IgG, heavy and light chains, as determined by immunoelectrophoresis against normal mouse serum. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
biological source: goat. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: secondary antibodies. clone: polyclonal. form: liquid. contains: ≤. 0.1% sodium azide as preservative. manufacturer/tradename: Calbiochem®. . storage condition: do not freeze. isotype: IgG. shipped in: wet ice. storage temp.: 2-8°C. target post-translational modification: unmodified. Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.- UPC:
- 41106600
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 401607-1ML