General description
IgG is the most abundant immunoglobulin and comprises two heavy chains and two light chains. Each molecule has two antigen binding sites. IgG has 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%). IgM constitutes about 10% of serum immunoglobulins. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells. Monomeric IgA constitutes 5-15 % of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces.
Specificity
Reacts with heavy and light chains of mouse IgM, IgG1, IgG2a, IgG2b, IgG3 and IgA. Absorbed for human sera and purified human paraproteins. Minimal cross reactivity with human immunoglobulins.
The antibody reacts with the heavy and light chains of mouse IgM, IgG1, IgG2a, IgG2b, IgG3 and IgA as demonstrated by ELISA and flow cytometry. Minimal cross reactivity with human immunoglobulins.
Immunogen
Epitope: Mouse IgM, IgG, IgA
Prepared from pepsin digest of mouse Ig (H+L).
Application
Detect Mouse IgM+IgG+IgA using this Goat anti-Mouse IgM+IgG+IgA Antibody, F(ab′)2, FITC conjugate, Species Adsorbed validated for use in ELISA & IF.
Immunofluorescence: ≤1 μg/106 cells.
ELISA
Optimal working dilutions must be determined by the end user.
Research Category
Secondary & Control Antibodies
Research Sub Category
Fragment Specific Secondary Antibodies
Physical form
Gel filtration chromatography
Purified by gel filtration chromatography of pepsin digested antibody. Liquid in 1.0 mL PBS/NaN3.
Storage and Stability
Maintain refrigerated at 2°-8°C under sterile conditions for up to twelve months from date of receipt. Do not freeze.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
- UPC:
- 12352207
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- AQ502F